Native intact analysis of a cysteine linked antibody drug conjugate

28th Mar 2022

Antibody-drug conjugates (ADCs) are an emerging class of biopharmaceutical combining the specific targeting nature of monoclonal antibodies (mAbs) with highly cytotoxic payloads. The field of conjugation strategies is rapidly evolving with innovative methods to control drug-antibody ratio (DAR). One of these such techniques is the conjugation of payloads to inter-chain cysteines after they have been reduced. This leads to reproducible even numbered species that can be tuned through levels of reduction. The antibody protein complex is held together through non-covalent interactions however this presents an analytical challenge for LC/MS as most methods rely upon denaturing reverse phase chromatography.

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