Mass spectrometry as a tool in ADC development

08th Aug 2023

Mass spectrometry (MS) is an appealing technique for protein characterisation as it provides additional information than purely optical techniques, with many existing chromatography methods being hyphenated with the appropriate buffers and columns. In addition, MS does not need chromatographic separation of conjugated species to provide drug to antibody ratio (DAR) which can assist on difficult payloads. MS can go further in localising specific residues of conjugation when combined with enzymatic digestions.

In the below, we outline a series of mass spectrometry approaches for characterising various conjugation lots to ensure comparability.

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Scientist Snapshot | Chris Nortcliffe, Mass Spectrometry Manager