A standardised LC-MS workflow for rapid DAR determination of thiol-conjugated ADCs across a variety of toxin linkers
18th Jul 2023
Fast, reliable and generic methods are required for drug to antibody ratio (DAR) determination in an ADC development laboratory. In an effort to establish such a method, we have expanded on a previously developed “middle-up” workflow for determination of DAR by liquid chromatography- mass spectrometry (LC-MS). We demonstrate application of the workflow to a range of stochastically conjugated ADCs with DARs from 1 – 8, incorporating auristatin, α-amanitin, duocarmycin, PBD dimer and DX8951 payloads.
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